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| Ramachandran,Sumitra; Patel,Anil K.; Nampoothiri,Kesavan Madhavan; Chandran,Sandhya; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok. |
| Solid-state fermentation was carried out for the production of alpha-amylase using Aspergillus oryzae. Different oil cakes such as coconut oil cake (COC) sesame oil cake (SOC), groundnut oil cake (GOC), palm kernel cake (PKC) and olive oil cake (OOC) were screened to be used as substrate for the enzyme production and also compared with wheat bran (WB). GOC was found to be the best producer of the enzyme among these. Combination of WB and GOC (1:1) resulted higher enzyme titres than the individual substrates. Maximum amount of enzyme (9196 U/gds) was obtained when SSF was carried out using WB + GOC, having initial moisture of 64% and supplemented with lactose and ammonium nitrate (1% each) at 30ºC for 72h using 2 mL spore suspension (6x10(7)spores/ml).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpha amylase; Oil cakes; Solid-state fermentation; Partial purification; Aspergillus oryzae. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200019 |
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| Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud. |
| This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,... |
| Tipo: Text |
Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf |
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| Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane. |
| To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to... |
| Tipo: Text |
Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf |
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| Huvet, Arnaud; Daniel, Jean-yves; Quere, Claudie; Dubois, Solene; Prudence, Marie; Van Wormhoudt, A; Sellos, D; Samain, Jean-francois; Moal, Jeanne. |
| A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to estimate tissue-specific expression of two amylase genes in five different tissues of the Pacific oyster Crassostrea gigas and the experimental effects of food level on the cellular, enzymatic and gene expression changes of the digestive gland. Results indicated that both genes were transcribed at different levels depending on tissue type. A strong preferential expression of both amylase genes was observed in the digestive gland leading to high amylase activities, in accordance with the digestive function of this organ. Traces of gene expression and activity were observed in other analysed tissues. These appeared higher in labial palps than in other... |
| Tipo: Text |
Palavras-chave: Oyster; Gene expression; Food regulation; Digestive enzyme activity; Alpha amylase. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-394.pdf |
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